17-OH-Progesterone ELISA

Background:The steroid hormone 17-OH-Progesterone (17-OHP) is produced in the adrenal cortex and in the gonads. Gestagenic effects exerted by 17-OHP are small. Nevertheless, this hormone is of clinical significance because it represents the ultimate precursor of 11β-desoxycortisol (compound S, CpS). CpS is formed by hydroxylation of the carbon atom C 21 of 17-OHP. Enzyme activity of 21-hydroxylase in the adrenal cortex may thus be monitored by analyzing the level of 17-OHP in the blood.

Description:
The 17-OH-Progesterone ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and enzyme-labeled antigen compete for the binding sites of antibodies coated onto the wells. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of 17-OHP in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of 17-OHP in the sample. The enzymatic reaction is stopped by addition of stop solution and the optical density (OD) is measured. A standard curve is constructed by plotting OD values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8 (break-apart) strips;
Calibrator: 0.1 - 0.4 - 1.6 - 6.5 - 25 ng/mL;
Microtiter plate reader at 450 nm;
Assay range: 0.1 - 25 ng/mL;
Analytical Sensitivity: 0.022 ng/mL;
IVD