Adrenaline Research ELISA

Description:
Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine). Flexible test system for various biological sample types and volumes. Adrenaline (epinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Product features:
The kit contains reagents for 96 determinations;

Microtiter plate reader at 450 nm and if possible 620-650 nm;
Analytical sensitivity: 3.3 pg/mL;
IVD