BACE1 Assay Kit

Background: Neuropathologically Alzheimer’s disease (AD) is characterized by widespread deposition of amyloid β protein (Aβ) in the cerebral cortex, and since the intracerebral accumulation of Aβ is highly specific for AD and the gene mutation responsible for familial amyloidosis causes Aβ to increase, Aβ is thought to be of extremely great significance in the pathogenetic mechanism of AD. Aβ is generated by proteolysis of the Aβ precursor protein (APP), a type I membrane protein. The enzyme that cleaves it at the N-terminal is called β secretase, and it is regarded as being the protease that acts at the initial step in the production of Aβ. The major β secretase has been identified as β-site APP Cleaving Enzyme 1 (BACE1), and BACE2 has been isolated as its homologue. Since increased BACE1 activity has also been reported in the brain of patients with sporadic AD, BACE1 inhibitors have been attracting attention as therapeutic agents for AD.

Description:
This kit is a solid phase sandwich ELISA using 2 kinds of high specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is in proportional to the quantities of BACE1.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 0,16 ng/mL