BactoReal® Kit Bartonella spp.

Detection of the gltA gene of Bartonella clarridgeiae, Bartonella elizabethae, Bartonella grahamii, Bartonella henselae, Bartonella koehlerae, Bartonella quintana, Bartonella volans and Bartonella washoensis

Background: Bartonella species are Gramnegative bacteria. In addition to dogs and cats, numerous domestic and wild animals, including bovine, canine, and rodent species as well as humans can serve as a reservoir host for various Bartonella species. Cats may harbor B. henselae, B. clarridgeiae and rarely other Bartonella species. Dogs may carry Bartonella vinsonii subsp. berkhoffii, Bartonella henselae and others.

Description: BactoReal® Kit Bartonella spp. uses real-time PCR for the detection of the gltA gene of Bartonella species. This kit is specific for B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and B. washoensis. Bartonella rochalimae, B. vinsonii and B. bovis are detected with very low sensitivity. Bartonella bacilliformis is not detected. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG)

Product Features:
- Amplification and detection: gltA gene of Bartonella spp.
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX