BactoReal® Kit Chlamydophila psittaci/abortus VET

Detection of the omp1 gene of Chlamydophila abortus and Chlamydophila psittaci

Background: Chlamydophila psittaci (previously classified as Chlamydia psittaci) is an intracellular bacterium that may cause endemic avian chlamydiosis in feral birds and domesticated poultry, epizootic outbreaks in mammals such as cattle, pigs, sheep and horses, and respiratory psittacosis in humans (also known as parrot fever and ornithosis). It is transmitted by inhalation, contact or ingestion among birds and to mammals. Chlamydophila psittaci is present in feces, nasal secretions, and feathers of infected birds. Chlamydophila abortus (previously classified as Chlamydia psittaci serotype 1) is one of the major infectious causes of abortion in sheep, cattle and goats worldwide by colonizing the placenta. Enzootic abortion in sheep is also known as ovine enzootic abortion (OEA) or enzootic abortion of ewes (EAE).

Description: BactoReal® Kit Chlamydophila psittaci/abortus uses real-time PCR for the detection of the omp1 gene of C. psittaci and C. abortus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: omp1 gene of Chlamydophila psittaci and Chlamydophila abortus
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX