BactoReal® Kit Enterococcus spp.

Detection of the 23S rRNA gene of Enterococcus species

Background: The genus Enterococcus includes more than 17 species and until 1984, Enterococcus species were classified as Group D Streptococcus. This genus belongs to lactic acid bacteria of the phylum Firmicutes. Enterococci are Grampositive cocci, they are facultative anaerobic organisms that can survive and grow in many environments. They are part of the normal intestinal flora of humans and animals but also found in soil, water, plants and insects. Enterococci are important pathogens responsible for serious infections. In cattle, enterococci have been associated with diarrhea in calves and bovine mastitis in dairy cattle.

Description: BactoReal® Kit Enterococcus spp. uses real-time PCR for the detection of the 23S rRNA gene of species of the genus Enterococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 23S rRNA gene of Enterococcus spp.
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX