BactoReal® Kit Streptococcus dysgalactiae

Detection and differentiation of 23S rRNA gene of Streptococcus dysgalactiae and Streptococcus canis and of 16S rRNA gene of Streptococcus species except Streptococcus canis

Background: Streptococcus dysgalactiae is a Grampositive bacterium belonging to the group C streptococci. It is known to cause clinically acute and subclinical mastitis in cows, ewes and goats, as well as polyarthritis.

Description: BactoReal® Kit Streptococcus dysgalactiae uses multiplex real-time PCR for the detection of S. dysgalactiae (S. dysgalactiae subsp. equisimilis and S. dysgalactiae subsp. dysgalactiae). This test is performed in a multiplex real-time PCR format to enable a differentiation between S. dysgalactiae from other Streptococcus species, especially S. canis. It is based on the amplification and detection of the 23S rRNA gene and 16S rRNA gene. In FAM channel the 23S rRNA gene of S. dysgalactiae and S. canis is detected. In VIC/HEX channel the 16S rRNA gene of several Streptococcus species (S. dysgalactiae, S. agalactiae, S. phocae, S. porcinus, S. didelphis) except that of S. canis is detected. Thus, samples showing a positive signal in FAM as well as in VIC/HEX channel are positive for S. dysgalactiae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 23S rRNA and 16S rRNA gene of Streptococcus dysgalactiae
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5