BactoReal® Kit Taylorella equigenitalis

Detection of the 16S rRNA gene of Taylorella equigenitalis

Background: T. equigenitalis is a Gramnegative bacterium and the causal agent of contagious equine metritis (CEM). CEM is an inflammation of the endometrium of mares, which usually results in temporary infertility. Taylorella equigenitalis is transmitted mainly during mating. It can also be spread by infected semen during artificial insemination.

Description: BactoReal® Kit Taylorella equigenitalis uses real-time PCR for the detection of the 16S rRNA gene of T. equigenitalis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 16S rRNA gene of Taylorella equigenitalis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX