BactoReal® Kit Tropheryma whipplei

Detection of the groEL gene of Tropheryma whipplei.

Background: Tropheryma whipplei is a Gram-positive bacterium very difficult to cultivate. It is a human pathogen causing Whipple's disease, which is a rare, chronic, systemic illness typically affecting white, middle-aged men. It causes intestinal malabsorption that leads to cachexia. In the majority of cases, multiple organ systems are affected. Symptoms include diarrhea and abdominal pain, joint pain, arthritis, and even dementia. It manifests in the body in various forms and can go undetected for decades before suddenly causing a fatal illness.

Description: BactoReal® Kit Tropheryma whipplei uses real-time PCR for the detection of the groEL gene of Tropheryma whipplei. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: groEL gene of Tropheryma whipplei
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or assay-only