BactoReal® Typing Kit Dichelobacter nodosus

Detection of Dichelobacter nodusus and differentiation between virulent and benign strains

Background:Footrot (infectious pododermatitis), is a hoof infection commonly found in sheep, goats, and cattle. It is responsible for the majority of cases of lameness in sheep. In sheep, there are two types of bacteria involved in causing footrot. Dichelobacter nodosus is the main cause of the disease, while Fusobacterium necrophorum is an associated pathogen. The two usually have a synergistic effect together. D. nodosus is an anaerobic, non-sporulating, Gram-negative bacterium. Virulent and benign strains of D. nodosus can be easily differentiated by real-time PCR. Virulent strains contain aprV2 genes encoding thermostable proteases, while benign strains harbor aprB2 genes encoding thermolabile proteases.
To differentiate between an D. nodosus and Fusobacterium necrophorum infection, ingenetix offers a separate real-time PCR test: BactoReal® Kit Footrot.

Description:BactoReal® Typing Kit Dichelobacter nodosus is a real-time PCR test for the qualitative detection of Dichelobacter nodusus and differentiation between virulent and benign strains. DNA of the aprV2 and aprB2 genomic regions is detected, respectively. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: DNA of aprV2 and aprB2 genomic regions of D. nodosus
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously