BactoReal® Typing Kit Paenibacillus larvae ERIC I

Detection of the plx1 gene of Paenibacillus larvae ERIC I.

Background:American foulbrood (AFB) is a bacterial brood disease of honey bees caused by the Gram-positive and spore-forming bacterium Paenibacillus larvae. The disease is transferred and initiated only by the spore stage of the bacterium. Young bee larvae are infected by spores, then the spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium produces many millions of spores which may remain dormant for many years. P. larvae ERIC I and II are frequently isolated from brood of AFB-infected colonies or from honey samples. The fast killing genotypes ERIC III, IV and V are obviously less prevalent in the context of AFB outbreaks since they have not been isolated in the recent years.
For the universal detection of AFB, independent of the ​Paenibacillus larvae​ genotype, ingenetix offers a separate real-time PCR Kit. ​

Description:BactoReal® Typing Kit Paenibacillus larvae ERIC I is a real-time PCR for the qualitative detection of the plx1 gene of Paenibacillus larvae ERIC I. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: plx1 gene of Paenibacillus larvae ERIC 1
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously