beta2-Microglobulin (beta2-M) ELISA

Enzyme immunoassay for the quantitative determination of ß2-Microglobulin in human serum.

INTENDED USE:For the direct quantitative determination of β2-Microglobulin by an enzyme immunoassay in human serum.

GENERAL INFORMATION:β2-Microglobulin (β2-M) is a single polypeptide chain containing 100 amino acids and is found on the surface of nucleated cells. β2 -M is continuously secreted into the circulatory system and therefore maintains a balanced serum level.

Clinical Trends:
- Decreases in the glomerular fi ltration rate gives rise to increases in the level of β2-M.
- β2 -M is a helpful marker in the diagnosis of kidney disease and active rheumatoid arthritis.
- Patients with acquired immune defi ciency syndrome (AIDS) show an increased level of β2-M.

β2-M has a low concentration in serum. We have found in a normal unselected population that in serum the highest level for β2 -M is 3.8 mg/L. The total number of serum samples tested was 92, showing little differences in the normal level in males, pre- and postmenopausal females. The average for male samples = 1.582 mg/L, for premenopausal female = 1.457 mg/L, for postmenopausal female = 1.608 mg/L and fi nally for young people = 1.13 mg/L.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (RT/shaker), 15-20 min (RT)
- Standard Range: 0.2 - 10 mg/l
- Sensitivity analytical: 0.1 mg/l
- Final Sample Volume: 20 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE