Condagene Salmonella spp.

Detection of Salmonella spp. in food products, animal feedstuff and environmental samples. Pre-enrichment broth according to ISO 6579 (recommended). For use in real time PCR instruments with FAM and ROX channels.

BACKGROUND: Salmonella spp. continues to be a major foodborne pathogen for animals and humans. In many countries, it is the leading cause of food-borne infections and outbreaks and due to the worldwide trade of food and feeding stuff, the risk of microbial infections for consumers increases. Culture-based methods used for Salmonella spp. detection often results in 4 - 6 days of isolation procedures.
In vitro amplification of DNA by the polymerase chain reaction (PCR) has become a powerful alternative in microbiological diagnostics due to its rapidity and accuracy. Today, there are approved methods for PCR based detection of Salmonella spp. unique DNA sequences, in particular using real time PCR and specific fluorescent probes.

PRODUCT DESCRIPTION: Condagene Salmonella spp. uses real-time PCR for the detection of Salmonella spp. in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. The carefully designed primers and probe ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Salmonella spp.
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Channel Pathogen: FAM, Channel IC: ROX