CRP canine ELISA

Background:The measurement of CRP is used for diagnosis and follow-up of inflammatory diseases. Alt- hough CRP is not disease-specific, the detection of an increased CRP level provides a valuable indication of the presence of inflammatory processes. CRP levels in serum/blood rise within hours after the onset of inflammation and reach a peak during the acute phase. As soon as the inflammation subsides, the CRP levels begin to decrease. As the erythrocyte sedimentation rate may be influenced by other physiological processes, the determination of CRP is a more reliable and more sensitive indicator of inflammation for: Rapid and easy detection of inflammatory processes; Monitoring of therapy (Immune-mediated inflammatory processes); Screening before and follow-up after surgery; Helpful as an aid in the diagnosis of infectious diseases; Useful as part of health checks – especially older dogs; Screening of health status e. g. before travelling or vaccination; Pregnancy; Cancer.

Description:
The CRP canine ELISA Kit is a 96 well immuno-capture ELISA product. Samples are incubated with a specific polyclonal antibody coated on the plate. After incubation, the unbound material is washed away. Then a HRP linked polyclonal antibody that specifically recognizes canine CRP is added to the wells. After a further incubation and washing step, a TMB substrate is added which reacts with the HRP and resulting in a concentration-depended color level. The reaction is stopped with HCl and the plate is read using a plate reader at 450 nm. Color development is proportional to the amount of canine CRP in the sample.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8 (break-apart) strips;
Microtiter plate reader at 450 nm;
Detection limit: 29,8 ng/ml.