DDE-DDT ELISA Kit

Product Description

The DDE/DDT ELISA is an immunoassay for the quantitative and sensitive screening of DDE/DDT. This test is suitable for the quantitative and/or qualitative screening of DDE/DDT in water samples, including groundwater, surface water, and well water. For soil and other sample matrices, please contact technical services for application bulletins and/or specific matrix validation guidelines. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.

The test is a direct competitive ELISA based on the recognition of DDE/DDT by specific antibodies. DDE/DDT, when present in a sample, and a DDE/DDT-HRP analogue compete for the binding sites of anti-DDE/DDT antibodies in solution. The DDE/DDT antibodies are then bound by a second antibody (anti-mouse) immobilized on the wells of the microtiter plate.

After a washing step and the addition of the substrate solution, a colour signal is generated. The intensity of the blue colour is inversely proportional to the concentration of DDE/DDT present in the sample. The colour reaction is stopped after a specified time and the colour is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Kit Features

• Quantitative and qualitative screening
Sensitive screening and detection of DDE/DDT in water samples.

• Direct competitive ELISA
Based on the specific recognition of DDE/DDT by anti-DDE/DDT antibodies.

• Suitable for multiple water matrices
Designed for screening DDE/DDT in groundwater, surface water, and well water.

• Easy result evaluation
Colour development is measured using a standard ELISA reader, with sample concentrations determined from the standard curve.

• Additional matrices
For soil and other sample matrices, please contact technical services for specific application and matrix validation guidelines.

• Regulatory confirmation
Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.