Dioxins-Furans (TCDD) ELISA

Product Description: The Dioxin/Furan ELISA is an indirect immunoassay (ELISA) for the quantitative and sensitive detection of Dioxins and Furans in water, soil and sediment samples. If necessary, positive samples can be confirmed by instrumental analysis or other conventional methods.

The test is an indirect competitive ELISA that allows the sensitive detection of Dioxins and Furans. It is based on the recognition of Dioxins and Furans congeners by specific antibodies. Dioxins and Furans, when present in a sample, and a Dioxin/Furan-protein analogue immobilized on the plate compete for the binding sites of antibodies in solution.

After a washing step, a second antibody-HRP label is added. Following a further washing step and the addition of the substrate solution, a colour signal is generated. The intensity of the blue colour is inversely proportional to the concentration of Dioxin/Furan present in the sample. The colour reaction is stopped after a specified time and the colour is evaluated using an ELISA reader.

Kit Features

• Quantitative detection
Sensitive quantitative detection of Dioxins and Furans.

• Indirect competitive ELISA
Based on specific antibody recognition of Dioxin and Furan congeners.

• Suitable for multiple matrices
Validated for water, soil and sediment samples.

• Easy result evaluation
Colour development is measured using a standard ELISA reader.

• Confirmation of positive samples
Positive results can, where necessary, be confirmed using instrumental analysis or other conventional methods.