Glutamate ELISA

>Background:Glutamate, also known as glutamic acid, is one of the most important excitatory neurotransmitter in the central nervous system (CNS). It is released presynaptically and it binds postsynaptically to specific receptors for glutamate. The enzyme glutamic acid decarboxylase is able to convert L-glutamate in the CNS by decarboxylation to γ-aminobutyric acid (GABA), which acts as an inhibitory neurotransmitter.

Description:
Enzyme Immunoassay for the quantitative determination of L-glutamate in urine and various biological samples. After extraction and derivatisation Glutamate is quantitatively determined by ELISA. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized analyte concentrations in the standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigenantibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This product is not intended to clinical diagnoses.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Limit of detection: 0,17 µg/mL.