MycoReal Kit Batrachochytrium dendrobatidis

Detection of the ITS-1 region of Batrachochytrium dendrobatidis

Background: Batrachochytrium dendrobatidis, also known as the amphibian chytrid fungus, causes the disease chytridiomycosis in amphibians. It infects the keratinized skin of amphibians.

Description: MycoReal Kit Batrachochytrium dendrobatidis uses real-time PCR for the detection of the the ITS2 region of Batrachochytrium dendrobatidis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: ITS2 region of Batrachochytrium dendrobatidis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX