Napsin A ELISA

Enzyme immunoassay for the quantitative determination human Napsin A in serum, urine and cell culture supernatant.

INTENDED USE:This assay kit is capable for the quantitative determination human Napsin A in serum, urine and cell culture supernatant.

GENERAL INFORMATION:Napsin A (NAPSA) is a pepsin-like aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. Napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kDa. Immunohistochemical studies revealed high expression levels of Napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs. This ELISA kit can measure human Napsin A.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 24 h (4°C), 30 (4°C), 30 min (RT)
- Standard Range: 3.91 - 250 ng/ml
- Sensitivity analytical: 2.92 ng/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, cell culture supernatant, urine
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: RUO