ParoReal® Kit Acarapis woodi

Detection of the COX1 gene of Acarapis woodi.

Background:Acarapis woodi is an endoparasite that colonizes the trachea, respectively the respiratory system of bees. Acarapisosis is listed by the WOAH (World Organisation for Animal Health) as notifiable disease of honeybees and has been found worldwide. Fatal losses of entire apiaries because of high infestation are described. The mite spreads from bee to bee, or through swarming and beekeepers. Infected bees could be asymptomatic or show just a few signs, thus the disease is not easily recognized. Like other arthropod pathogens A. woodi is not easy visible by eyes. In early spring crawling and death bees in front of the hive could be the only sign of infection. Due to this fact and that mites are weakening the immunity of the hives in general, A. woodi might act also as a vector of other bacterial and viral pathogens.

Description:ParoReal® Kit Acarapis woodi is a real-time PCR test for the qualitative detection of the COX1 gene of Acarapis woodi. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: COX1 gene of Acarapis woodi
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously