PCBs Higher Chlorinated ELISA

Product Description: The PCB ELISA is an immunoassay for the quantitative and sensitive screening of Polychlorinated Biphenyls (PCBs). This test is suitable for the quantitative and/or qualitative screening of PCBs in water and soil samples (please refer to the appropriate technical bulletins for additional matrix procedures). Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.

The test is a direct competitive ELISA based on the recognition of Polychlorinated Biphenyls by specific antibodies. The PCBs, when present in a sample, and a PCB-HRP analogue compete for the binding sites of anti-PCB antibodies in solution. The PCB antibodies are then bound by a second antibody immobilized on the wells of the microtiter plate.

After a washing step and the addition of the substrate solution, a colour signal is generated. The intensity of the blue colour is inversely proportional to the concentration of PCBs present in the sample. The colour reaction is stopped after a specified time and the colour is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Kit Features

• Quantitative and qualitative screening
Sensitive screening of Polychlorinated Biphenyls (PCBs).

• Direct competitive ELISA
Based on the specific recognition of PCBs by anti-PCB antibodies.

• Suitable for multiple matrices
Suitable for PCB screening in water and soil samples.

• Easy result evaluation
Colour development is measured using a standard ELISA reader, with sample concentrations determined from the standard curve.

• Regulatory confirmation
Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.