Phthalates ELISA

Product Description: The Phthalates ELISA is an immunoassay for the detection of Phthalates in surface water. This test is suitable for the quantitative and/or qualitative detection of Phthalates in contaminated samples. Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods.

The test is a direct competitive ELISA based on the recognition of Phthalates by specific antibodies. Phthalates, when present in a sample, and a Phthalates-enzyme conjugate compete for the binding sites of anti-Phthalates antibodies in solution. The Phthalates antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the microtiter plate.

After a washing step and the addition of the substrate solution, a colour signal is produced. The intensity of the blue colour is inversely proportional to the concentration of Phthalates present in the sample. The colour reaction is stopped after a specified time and the colour is evaluated using a microplate ELISA photometer. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Kit Features

• Quantitative and qualitative detection
Sensitive screening and detection of Phthalates in contaminated samples.

• Direct competitive ELISA
Based on the specific recognition of Phthalates by anti-Phthalates antibodies.

• Suitable for surface water
Designed for the detection of Phthalates in surface water samples.

• Easy result evaluation
Colour development is measured using a microplate ELISA photometer, with sample concentrations determined from the standard curve.

• Confirmation of positive samples
Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods.