PREraser BACGene

In microbiological workflows with PCR (Polymerase Chain Reaction) as a detection method, free bacterial DNA in enrichments can sometimes cause problems in the interpretation of results. PCR does not distinguish between DNA from living and dead bacteria. Since PCR is a highly sensitive method and DNA a rather stable molecule, the dilution factors introduced in the workflow (e.g. enrichment) do not always solve the problem. Free DNA from dead bacteria can thus be detected by PCR and might produce misleading results (culturally unconfirmed PCR-positives).

The PREraser BACGene kit targets free DNA from samples prior to the lysis step by a simple enzymatic digestion and thus reduces PCR signals from free DNA. It can be combined with the BACGene real-time PCR kits for Salmonella, Listeria and E.coli/STEC. For elimination of free DNA from Listeria, Salmonella, and E. coli in selected matrices, for sample pre-treatment after enrichment and prior to lysis according to the respective protocols.

Kit with two formats:
- PREraser BACGene (1 mL) (5123222301)
- PREraser BACGene (1 mL) x 10 (5123222311)

For use with:
- BACGene Salmonella spp. (5123221801)
- BACGene Listeria Multiplex (5123221901)
- BACGene Listeria spp. (5123222101)
- BACGene Listeria monocytogenes (5123222001)
- BACGene E. coli O157:H7 (5123222701)
- BACGene Mplex STEC Screen (5123223001); (5123223101); (5123223201)
- BACGene Mplex STEC SEROtype1 and SEROtype2

AOAC and AFNOR Certification