Real Time PCR Detection Kit Clostridium perfringens Epsilon toxin

The Real Time PCR detection Kit Clostridium perfringens Epsilon toxin comprises a series of specific targeted reagents designed for Clostridium perfringens Epsilon toxin detection by using qPCR.

GENERAL INFORMATION:Clostridium perfringens (formerly known as C. welchii, or Bacillus welchii) is a spore-forming Gram-positive bacterium. It is found in many environmental sources as well as in the intestines of humans and animals. C. perfringens is responsible of food poisoning, gastrointestinal disease, gas gangrene and related necrotic conditions in humans and other mammals. This bacterium produces 4 major toxins (named Alpha, Beta, Epsilon and Iota). Epsilon toxins is produced by type B and type D strains of C. perfringens and is most commonly isolated from animals, particularly sheep, goats, and cattle, but rarely from humans. Epsilon toxin creates pores in tissues, resulting in leaked potassium ions and fluid leakage, which leads to greater complications, giving way to the symptoms associated with C. perfringens infection. Two common diseases seen in animals as a result from type B and D strains of C. perfringens are lamb dysentery and pulpy kidney disease.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control