Real Time PCR Detection Kit Haemotrophic mycoplasmas Panel

The Real Time PCR detection Kit Haemotrophic mycoplasmas Panel (Mycoplasma haemofelis/haemocanis, Mycoplasma haemominutum (Candidatus), Mycoplasma turicensis (Candidatus) and Mycoplasma haematoparvum (Candidatus)) comprises a series of specific targeted reagents designed for Mycoplasma haemofelis/haemocanis, Candidatus Mycoplasma haematoparvum, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis detection by using qPCR.

GENERAL INFORMATION:Mycoplasma is a bacterial genus that lacks a cell wall surrounding the cell membrane. Since there is no murein present in these bacteria, they are naturally resistant to antibiotics that target cell wall synthesis. They are able to colonize the outside of erythrocytes and infect a wide range of vertebrate hosts. Infection with haemotrophic mycoplasmas is often asymptomatic, but it can potentially lead to massive bacteraemia of red blood cells and severe haemolytic anaemia (haemobartonellosis). The bacteria can be transmitted from one animal to another by blood-feeding arthropods, such as fleas and ticks. Molecular detection by qPCR is the most sensitive and specific way of detecting bacteria in the blood of infected animals having acute or latent infections. Mycoplasma haemocanis (formerly Haemobartonella canis) and Candidatus Mycoplasma haemotoparvum are parasitic bacteria capable of infecting immunocompromised or splenectomised dogs mainly. Mycoplasma haemofelis (formerly Haemobartonella felis), Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis are the three haemoplasma species identified to affect domestic cats.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control