SENSIColumn IAC Biotin

Fast and accurate content determination of Biotin in Vitamin Tablets, Liquid Vitamin Preparations, Cell Culture Extracts, among others, by combination of Affinity Chromatography, HPLC-FLD and Post Column Derivatization with Fluorescein-Streptavidin Conjugate as Fluorophor.

Many methods of biotin determination based on HPLC-UV detection show either low sensitivity or low selectivity if problematic matrices are applied depending on the factor of dilution of the matrices.
This method of content determination of Biotin combines the high selectivity of affinity columns with its potential to concentrate elute and the very sensitive detection of biotin by post column labelling with fluorescein-treptavidin conjugate.

Related Kits:
- EFSA3175:SENSIColumn IAC Biotin;
- BTCA3185:SENSIColumn IAC Vitamin B12;
- BTFS3195:SENSIColumn IAC Folic Acid