Shrimp Alkaline Phosphatase (1U/uL)

Heat-labile multipurpose alkaline phosphatase that catalyzes the dephosphorylation of DNA, RNA and nucleotides. This recombinant enzyme replaces native SAP because it is much more stable at room temperature and is available at higher concentrations.

PRODUCT: Recombinant Shrimp Alkaline Phosphatase (rSAP) is a heat-labile multipurpose alkaline phosphatase that catalyzes the dephosphorylation of DNA, RNA, and nucleotides. This recombinant enzyme replaces native SAP because it is much more stable at room temperature and is available at higher concentrations. For activity, rSAP requires magnesium (>1mM) and is tolerant to a wide variety of buffer conditions (salt, pH, etc), and thus can be added directly to many molecular biology buffers, including PCR mixtures and most restriction enzyme buffers. rSAP is completely inactivated by incubation at 65ºC for 5 minutes.
Application: Dephosphorylation of dNTPs from PCR reaction mixtures prior to DNA sequencing (typically in a PCR Clean-up protocol1 in combination with the use of Exonuclease I (Exo I)). Dephosphorylation of DNA prior to end-labelling (using T4 Polynucleotide Kinase). Dephosphorylation of vectors (plasmids) during cloning, in order to prevent recircularization during ligation reaction (using T4 DNA Ligase).