Toxoplasma gondii IgG ELISA

Enzyme immunoassay for the quantitative determination of IgG class antibodies against Toxoplasma gondii in human serum or plasma.

INTENDED USE:The Toxoplasma gondii IgG ELISA is intended for the determination of IgG class antibodies against Toxoplasma gondii in human serum or plasma (citrate, heparin). For research use only – Not for use in diagnostic procedures.

GENERAL INFORMATION:The immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (37°C), 30 min (RT), 15 min (RT/dark)
- Standard Range: 50 - 200 IU/ml
- Sensitivity analytical: 1.04 IU/ml
- Final Sample Volume: 100 µl
- Sample Type: serum, plasma (citrate, heparin)
- Sample Pretreatment: 1:101 diluted
- Isotope / Substrate: TMB 450 nm
- Regulatory Status: CE0483/RUO