Trifluralin ELISA Kit

Product Description

The Trifluralin ELISA is an immunoassay for the detection of Trifluralin in surface water, fish, and shrimp samples. This test is suitable for the quantitative and/or qualitative detection of Trifluralin in contaminated samples. Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods.

The test is a direct competitive ELISA based on the recognition of Trifluralin by specific antibodies. Trifluralin, when present in a sample, and a Trifluralin-enzyme conjugate compete for the binding sites of anti-Trifluralin antibodies in solution. The Trifluralin antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the plate.

After a washing step and the addition of the substrate solution, a colour signal is produced. The intensity of the blue colour is inversely proportional to the concentration of Trifluralin present in the sample. The colour reaction is stopped after a specified time and the colour is evaluated using a microplate ELISA photometer. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Kit Features

• Quantitative and qualitative detection
Sensitive detection and screening of Trifluralin in contaminated samples.

• Direct competitive ELISA
Based on the specific recognition of Trifluralin by anti-Trifluralin antibodies.

• Multiple sample matrices
Suitable for surface water, fish, and shrimp samples.

• Easy result evaluation
Colour development is measured using a microplate ELISA photometer, with sample concentrations determined from the standard curve.

• Quantitative results
Sample concentrations are determined by interpolation using the standard curve constructed with each assay run.

• Confirmation of positive samples
Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods.