ViroReal® Kit Bovine Coronavirus

Detection of RNA of the N gene of BCoV

Background: Bovine coronavirus (BCoV) is a member of the genus Coronavirus (Betacoronavirus), family Coronaviridae. The BCoV virion is enveloped and spherical in shape. The genome is a single-stranded, positive-sense RNA molecule. BCoV causes diarrhea in adult cattle (Winter Dysentery) and wild ruminants, as well as enteric and respiratory diseases in calves. Winter Dysentery is characterized by high morbidity (50-100%) and low mortality (1-2%). The highest incidence is between November and April when cattle are kept in close confinement. In calves, morbidity and mortality are high. BCoV has been found in cattle worldwide.

Description: ViroReal® Kit Bovine Coronavirus uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid protein gene (N gene) of the BCoV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: N gene of Bovine Coronavirus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX