ViroReal® Kit Chronic Bee Paralysis Virus

Detection of the RNA of the RdRp gene of chronic bee paralysis virus.

Background:Chronic bee paralysis virus (CBPV) is an enveloped virus with a genome consisting of three single plus-stranded RNA molecules. It commonly affects the honeybee Apis mellifera. The unclassified virus is phylogenetically placed between the Nodaviridae (insect and fish viruses) and Tombusviridae (plant viruses) family. It is suggested that CBPV is the prototype of a new family of positive single-stranded RNA viruses. Infections were rather unusual but a continuous increase of incidents worldwide was reported recently. The very characteristic symptoms like inability to fly, trembling, ataxia, and shiny hairless bodies could even lead to collapses of the bee colonies. Infected symptomatic bees, mostly adults, die within a few days.

Description:ViroReal® Kit Chronic Bee Paralysis Virus is a real-time PCR for the qualitative detection of the RNA of the RdRp gene of chronic bee paralysis virus. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: RdRp gene of Chronic Bee Paralyis Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)