ViroReal® Kit classical swine fewer (CSF) Virus

Detection of RNA of the 3´UTR of classical swine fewer virus (KSPV)

Background: The CSF (classical swine fever virus) virus is a single strain, positive-sense ((+)sRNA) RNA virus belonging to the genus Pestivirus in the family Flaviviridae. This virus is closely related to ruminant pestiviruses that cause bovine viral diarrhoea (BVDV) and border disease (BDV) of sheep, both of which can also infect pigs naturally causing reproductive problems. The CSF virus is the infectious agent of classical swine fever or hog cholera (also sometimes called pig plague based on the German word “Schweinepest”), which is a highly contagious disease of swine. Swine fever virus causes fever, skin lesions, convulsions, and usually death. The effect of different CSF virus strains varies widely, leading to a wide range of clinical signs. There is only one serotype of the virus and attenuated vaccines are available.

Description: ViroReal® Kit CSF Virus uses one-step reverse transcription RT real-time PCR for the detection of the 5´UTR of the CSF virus genome. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: 5´UTR of CSF Virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX