ViroReal® Kit Pseudorabiesvirus (SHV-1)

Detection of the glycoprotein B gene of pseudorabiesvirus (suid herpesvirus 1, aujeszk´s disease virus)

Background: Pseudorabiesvirus (PRV) is a swine DNA herpesvirus of the Alphaherpesvirinae subfamily. It is also known as suid herpesvirus 1 (SHV-1), or by its original name, Aujeszky's disease virus. The pig is the principal reservoir host, but the virus may also infect other mammals like cattle, little ruminants, carnivores and rodents. It is not pathogenic for humans. In swine, Aujeszky’s disease shows itself under three forms: a nervous form, a respiratory form or a genital form. In latently infected pigs, pseudorabiesvirus persists primarily in the trigeminal or sacral ganglia and the tonsils. In other mammals, it causes infection of the central nervous system with neurologic behavioral changes leading very quickly to death.

Description: ViroReal® Kit Pseudorabiesvirus uses real-time PCR for the detection of the glycoprotein B gene of SHV-1. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: glycoprotein B gene of Pseudorabiesvirus (SHV-1)
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX