The PREraser BACGene kit removes free DNA prior to the lysis step through enzymatic digestion, significantly reducing PCR signals and offering a simpler alternative to methods using propidium monoazide (PMA) or ethidium monoazide (EMA). For elimination of free DNA from Listeria, Salmonella, and E. coli in selected matrices, for sample pre-treatment after enrichment and prior to lysis according to the respective protocols.